m avium chester Search Results


m avium  (ATCC)
94
ATCC m avium
M Avium, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m avium atcc 35718  (ATCC)
94
ATCC m avium atcc 35718
M Avium Atcc 35718, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m avium subsp  (ATCC)
95
ATCC m avium subsp
Strains and plasmids used in this study
M Avium Subsp, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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virulent m av strain mac 101  (ATCC)
96
ATCC virulent m av strain mac 101
Strains and plasmids used in this study
Virulent M Av Strain Mac 101, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mycobacterium avium subsp hominissuis  (ATCC)
93
ATCC mycobacterium avium subsp hominissuis
Strains and plasmids used in this study
Mycobacterium Avium Subsp Hominissuis, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mycobacterium chelonae m chelonae atcc  (ATCC)
93
ATCC mycobacterium chelonae m chelonae atcc
Strains and plasmids used in this study
Mycobacterium Chelonae M Chelonae Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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6003 m avium subsp avium chicken atcc 35713  (ATCC)
93
ATCC 6003 m avium subsp avium chicken atcc 35713
Strains and plasmids used in this study
6003 M Avium Subsp Avium Chicken Atcc 35713, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m avium spp avium  (DSMZ)
91
DSMZ m avium spp avium
Strains and plasmids used in this study
M Avium Spp Avium, supplied by DSMZ, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mycobacterium avium m intracellulare complex  (ATCC)
90
ATCC mycobacterium avium m intracellulare complex
Strains and plasmids used in this study
Mycobacterium Avium M Intracellulare Complex, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m avium chester  (ATCC)
93
ATCC m avium chester
Strains and plasmids used in this study
M Avium Chester, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Strains and plasmids used in this study

Journal:

Article Title: Characterization of Genetic Differences between Mycobacterium avium subsp. paratuberculosis Type I and Type II Isolates

doi: 10.1128/JCM.41.11.5215-5223.2003

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: Other template DNA used was from two nonpigmented bovine (lanes 1 and 2), two pigmented (lanes 3 and 4), three nonpigmented ovine (lanes 5 to 7), three nonpigmented caprine (lanes 8 to 10), and one nonpigmented human (lane 11) M. avium subsp . paratuberculosis isolate as well as four M. avium subsp . avium isolates (ATCC 35712, ATCC 25291, DSM 44158, and MAA S4; lanes 12 to 15). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 3. caption a7 Genetic organization of the loci pig-RDA10, pig-RDA20, and pig-RDA30 specific for M. avium subsp . paratuberculosis type I isolates.

Techniques: TA Cloning, Plasmid Preparation, Cloning

Primers used in this study

Journal:

Article Title: Characterization of Genetic Differences between Mycobacterium avium subsp. paratuberculosis Type I and Type II Isolates

doi: 10.1128/JCM.41.11.5215-5223.2003

Figure Lengend Snippet: Primers used in this study

Article Snippet: Other template DNA used was from two nonpigmented bovine (lanes 1 and 2), two pigmented (lanes 3 and 4), three nonpigmented ovine (lanes 5 to 7), three nonpigmented caprine (lanes 8 to 10), and one nonpigmented human (lane 11) M. avium subsp . paratuberculosis isolate as well as four M. avium subsp . avium isolates (ATCC 35712, ATCC 25291, DSM 44158, and MAA S4; lanes 12 to 15). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 3. caption a7 Genetic organization of the loci pig-RDA10, pig-RDA20, and pig-RDA30 specific for M. avium subsp . paratuberculosis type I isolates.

Techniques: Sequencing

Molecular data for the M. avium subsp. paratuberculosis subspecies- and type I-specific fragments

Journal:

Article Title: Characterization of Genetic Differences between Mycobacterium avium subsp. paratuberculosis Type I and Type II Isolates

doi: 10.1128/JCM.41.11.5215-5223.2003

Figure Lengend Snippet: Molecular data for the M. avium subsp. paratuberculosis subspecies- and type I-specific fragments

Article Snippet: Other template DNA used was from two nonpigmented bovine (lanes 1 and 2), two pigmented (lanes 3 and 4), three nonpigmented ovine (lanes 5 to 7), three nonpigmented caprine (lanes 8 to 10), and one nonpigmented human (lane 11) M. avium subsp . paratuberculosis isolate as well as four M. avium subsp . avium isolates (ATCC 35712, ATCC 25291, DSM 44158, and MAA S4; lanes 12 to 15). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 3. caption a7 Genetic organization of the loci pig-RDA10, pig-RDA20, and pig-RDA30 specific for M. avium subsp . paratuberculosis type I isolates.

Techniques:

PCR analysis of M. avium subsp. paratuberculosis isolates of different host groupings by using the internal primer pair I130A/131A and Tsp45I restriction of the PCR product RD I130. The DNA template used was derived from three nonpigmented bovine (lanes 1 to 3), three pigmented ovine (lanes 4 to 6), three nonpigmented ovine (lanes 7 to 9), three nonpigmented caprine (lanes 10 to 12), and one nonpigmented human M. avium subsp. paratuberculosis isolate (lane 13) as well as the M. avium subsp. avium ATCC 25291 isolate (lane 14). (a) The PCR with the primers I130A/I131A resulted in products of different sizes for the M. avium subsp. paratuberculosis type I and type II isolates. (b) The PCR product RD I130 of the M. avium subsp. paratuberculosis type I isolates was cut into two fragments by digestion with Tsp45I. The arrows to the left indicate the size of the corresponding PCR products.

Journal:

Article Title: Characterization of Genetic Differences between Mycobacterium avium subsp. paratuberculosis Type I and Type II Isolates

doi: 10.1128/JCM.41.11.5215-5223.2003

Figure Lengend Snippet: PCR analysis of M. avium subsp. paratuberculosis isolates of different host groupings by using the internal primer pair I130A/131A and Tsp45I restriction of the PCR product RD I130. The DNA template used was derived from three nonpigmented bovine (lanes 1 to 3), three pigmented ovine (lanes 4 to 6), three nonpigmented ovine (lanes 7 to 9), three nonpigmented caprine (lanes 10 to 12), and one nonpigmented human M. avium subsp. paratuberculosis isolate (lane 13) as well as the M. avium subsp. avium ATCC 25291 isolate (lane 14). (a) The PCR with the primers I130A/I131A resulted in products of different sizes for the M. avium subsp. paratuberculosis type I and type II isolates. (b) The PCR product RD I130 of the M. avium subsp. paratuberculosis type I isolates was cut into two fragments by digestion with Tsp45I. The arrows to the left indicate the size of the corresponding PCR products.

Article Snippet: Other template DNA used was from two nonpigmented bovine (lanes 1 and 2), two pigmented (lanes 3 and 4), three nonpigmented ovine (lanes 5 to 7), three nonpigmented caprine (lanes 8 to 10), and one nonpigmented human (lane 11) M. avium subsp . paratuberculosis isolate as well as four M. avium subsp . avium isolates (ATCC 35712, ATCC 25291, DSM 44158, and MAA S4; lanes 12 to 15). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 3. caption a7 Genetic organization of the loci pig-RDA10, pig-RDA20, and pig-RDA30 specific for M. avium subsp . paratuberculosis type I isolates.

Techniques: Derivative Assay

PCR analyses of M. avium subsp. paratuberculosis and M. avium subsp. avium isolates with the primer pair p19/p20, specific for pig-RDA10 (a); p21/p22, specific for pig-RDA20 (b); p23/p24, specific for pig-RDA30 (c); ISMav2 I/ISMav2 II, specific for M. avium subsp. paratuberculosis (d); MK5/MK6, specific for IS900 of M. avium subsp. paratuberculosis (e); and (f) MK7/MK8, specific for IS901 of M. avium subsp. avium. The template DNA used was derived from the nonpigmented type II driver strain (lane D) and the pigmented type I tester strain (lane T). Other template DNA used was from two nonpigmented bovine (lanes 1 and 2), two pigmented (lanes 3 and 4), three nonpigmented ovine (lanes 5 to 7), three nonpigmented caprine (lanes 8 to 10), and one nonpigmented human (lane 11) M. avium subsp. paratuberculosis isolate as well as four M. avium subsp. avium isolates (ATCC 35712, ATCC 25291, DSM 44158, and MAA S4; lanes 12 to 15).

Journal:

Article Title: Characterization of Genetic Differences between Mycobacterium avium subsp. paratuberculosis Type I and Type II Isolates

doi: 10.1128/JCM.41.11.5215-5223.2003

Figure Lengend Snippet: PCR analyses of M. avium subsp. paratuberculosis and M. avium subsp. avium isolates with the primer pair p19/p20, specific for pig-RDA10 (a); p21/p22, specific for pig-RDA20 (b); p23/p24, specific for pig-RDA30 (c); ISMav2 I/ISMav2 II, specific for M. avium subsp. paratuberculosis (d); MK5/MK6, specific for IS900 of M. avium subsp. paratuberculosis (e); and (f) MK7/MK8, specific for IS901 of M. avium subsp. avium. The template DNA used was derived from the nonpigmented type II driver strain (lane D) and the pigmented type I tester strain (lane T). Other template DNA used was from two nonpigmented bovine (lanes 1 and 2), two pigmented (lanes 3 and 4), three nonpigmented ovine (lanes 5 to 7), three nonpigmented caprine (lanes 8 to 10), and one nonpigmented human (lane 11) M. avium subsp. paratuberculosis isolate as well as four M. avium subsp. avium isolates (ATCC 35712, ATCC 25291, DSM 44158, and MAA S4; lanes 12 to 15).

Article Snippet: Other template DNA used was from two nonpigmented bovine (lanes 1 and 2), two pigmented (lanes 3 and 4), three nonpigmented ovine (lanes 5 to 7), three nonpigmented caprine (lanes 8 to 10), and one nonpigmented human (lane 11) M. avium subsp . paratuberculosis isolate as well as four M. avium subsp . avium isolates (ATCC 35712, ATCC 25291, DSM 44158, and MAA S4; lanes 12 to 15). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 3. caption a7 Genetic organization of the loci pig-RDA10, pig-RDA20, and pig-RDA30 specific for M. avium subsp . paratuberculosis type I isolates.

Techniques: Derivative Assay

Genetic organization of the loci pig-RDA10, pig-RDA20, and pig-RDA30 specific for M. avium subsp. paratuberculosis type I isolates. The numbers given in the maps relate to the positions on the M. avium subsp. avium (TIGR strain 104, contig 3294) and M. avium subsp. paratuberculosis (UMN strain K-10, contig 16) genomes. (a) pig-RDA10 locus. The sequence identified is also present in the 16K locus present in M. avium subsp. avium (top) and M. avium subsp. paratuberculosis type I (middle) but is absent from M. avium subsp. paratuberculosis type II (bottom). The 16K locus is flanked by a 0.9-Mbp sequence (designated A) present in M. avium subsp. avium and partially present in M. avium subsp. paratuberculosis but is oriented in the opposite direction (indicated by an arrow). These sequences are bounded by regions (designated B, C, and D) which are interrupted by IS900 locus 15 in both M. avium subsp. paratuberculosis type I and type II isolates. A putative organization of the locus in M. avium subsp. paratuberculosis type I strains is depicted in the middle map. (b) pig-RDA20 locus. The sequence identified is also present in the 197K locus specific for M. avium subsp. avium (top). In M. avium subsp. paratuberculosis the same flanking regions embrace the 19Klocus specific for M. avium subsp. paratuberculosis (middle). The probable organization of the locus in M. avium subsp. paratuberculosis type I strains is depicted in the bottom map. (c) pig-RDA30 locus. The sequence identified is also present in the 27K locus specific for M. avium subsp. avium (top) flanked by a transposase (designated 1K) and containing an additional truncated 1K region. In M. avium subsp. paratuberculosis the 27K locus is missing (middle). The probable organization of the locus in M. avium subsp. paratuberculosis type I strains is depicted in the bottom map. The arrows are numbered to indicate the positions of the primers used (Table ​(Table44).

Journal:

Article Title: Characterization of Genetic Differences between Mycobacterium avium subsp. paratuberculosis Type I and Type II Isolates

doi: 10.1128/JCM.41.11.5215-5223.2003

Figure Lengend Snippet: Genetic organization of the loci pig-RDA10, pig-RDA20, and pig-RDA30 specific for M. avium subsp. paratuberculosis type I isolates. The numbers given in the maps relate to the positions on the M. avium subsp. avium (TIGR strain 104, contig 3294) and M. avium subsp. paratuberculosis (UMN strain K-10, contig 16) genomes. (a) pig-RDA10 locus. The sequence identified is also present in the 16K locus present in M. avium subsp. avium (top) and M. avium subsp. paratuberculosis type I (middle) but is absent from M. avium subsp. paratuberculosis type II (bottom). The 16K locus is flanked by a 0.9-Mbp sequence (designated A) present in M. avium subsp. avium and partially present in M. avium subsp. paratuberculosis but is oriented in the opposite direction (indicated by an arrow). These sequences are bounded by regions (designated B, C, and D) which are interrupted by IS900 locus 15 in both M. avium subsp. paratuberculosis type I and type II isolates. A putative organization of the locus in M. avium subsp. paratuberculosis type I strains is depicted in the middle map. (b) pig-RDA20 locus. The sequence identified is also present in the 197K locus specific for M. avium subsp. avium (top). In M. avium subsp. paratuberculosis the same flanking regions embrace the 19Klocus specific for M. avium subsp. paratuberculosis (middle). The probable organization of the locus in M. avium subsp. paratuberculosis type I strains is depicted in the bottom map. (c) pig-RDA30 locus. The sequence identified is also present in the 27K locus specific for M. avium subsp. avium (top) flanked by a transposase (designated 1K) and containing an additional truncated 1K region. In M. avium subsp. paratuberculosis the 27K locus is missing (middle). The probable organization of the locus in M. avium subsp. paratuberculosis type I strains is depicted in the bottom map. The arrows are numbered to indicate the positions of the primers used (Table ​(Table44).

Article Snippet: Other template DNA used was from two nonpigmented bovine (lanes 1 and 2), two pigmented (lanes 3 and 4), three nonpigmented ovine (lanes 5 to 7), three nonpigmented caprine (lanes 8 to 10), and one nonpigmented human (lane 11) M. avium subsp . paratuberculosis isolate as well as four M. avium subsp . avium isolates (ATCC 35712, ATCC 25291, DSM 44158, and MAA S4; lanes 12 to 15). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 3. caption a7 Genetic organization of the loci pig-RDA10, pig-RDA20, and pig-RDA30 specific for M. avium subsp . paratuberculosis type I isolates.

Techniques: Sequencing

Primers for the PCR analyses of the M. avium subsp. avium 16K locus and the M. avium subsp. paratuberculosis C locus

Journal:

Article Title: Characterization of Genetic Differences between Mycobacterium avium subsp. paratuberculosis Type I and Type II Isolates

doi: 10.1128/JCM.41.11.5215-5223.2003

Figure Lengend Snippet: Primers for the PCR analyses of the M. avium subsp. avium 16K locus and the M. avium subsp. paratuberculosis C locus

Article Snippet: Other template DNA used was from two nonpigmented bovine (lanes 1 and 2), two pigmented (lanes 3 and 4), three nonpigmented ovine (lanes 5 to 7), three nonpigmented caprine (lanes 8 to 10), and one nonpigmented human (lane 11) M. avium subsp . paratuberculosis isolate as well as four M. avium subsp . avium isolates (ATCC 35712, ATCC 25291, DSM 44158, and MAA S4; lanes 12 to 15). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 3. caption a7 Genetic organization of the loci pig-RDA10, pig-RDA20, and pig-RDA30 specific for M. avium subsp . paratuberculosis type I isolates.

Techniques: Sequencing

Primers for the PCR analyses of the M. avium subsp. avium 197K locus and the M. avium subsp. paratuberculosis 19K locus

Journal:

Article Title: Characterization of Genetic Differences between Mycobacterium avium subsp. paratuberculosis Type I and Type II Isolates

doi: 10.1128/JCM.41.11.5215-5223.2003

Figure Lengend Snippet: Primers for the PCR analyses of the M. avium subsp. avium 197K locus and the M. avium subsp. paratuberculosis 19K locus

Article Snippet: Other template DNA used was from two nonpigmented bovine (lanes 1 and 2), two pigmented (lanes 3 and 4), three nonpigmented ovine (lanes 5 to 7), three nonpigmented caprine (lanes 8 to 10), and one nonpigmented human (lane 11) M. avium subsp . paratuberculosis isolate as well as four M. avium subsp . avium isolates (ATCC 35712, ATCC 25291, DSM 44158, and MAA S4; lanes 12 to 15). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 3. caption a7 Genetic organization of the loci pig-RDA10, pig-RDA20, and pig-RDA30 specific for M. avium subsp . paratuberculosis type I isolates.

Techniques: Sequencing